基于细胞电生理检测的在体及离体微电极设计仿真

Design and Simulation of Extracellular Microelectrode Array In-Vitro and In-Vivo

生物传感器的微型和集成化为细胞电生理研究提供了强有力的手段。当前国内电极阵列设计的量化分析工具尚为欠缺。着重采用有限元工具分析电极形状对输出影响,对相同设计参数下圆形与矩形电极的电场分布进行比较;采用Monte-Carlo模型判断细胞与电极耦合的有效性,结果显示表面处理时,大分子生物素宜最后贴附处理以增大有效贴附率;采用SPICE工具对细胞并行电生理检测串扰性进行分析,结果说明细胞与电极的耦合性比阵列间距和数目对串扰影响更大。从而为有源电极的集成设计、细胞-电极耦合有效性和电生理测试提供量化分析工具。

Microelectrode arrays and probes provide methods down to cellular level by micromation and inte- gration. Though domestic electrodes are useful as a gain of information on the micro-circuit neuronal dynamics and signal propagation,more quantified evaluating analysis should be applied. In this respect, electrical properties of microarrays were analyzed by FEM tools to. compare electric field distribution of circle electrode with that of rectangular electrode, Monte-Carlo statistical model to demonstrate the surface immobilized effects,and SPICE tools to simulate how possible artifacts can be observed on nearby microelectrodes. The simulating results showed that big moleculars were better immobilized at last step of surface immobilization for a larger surface coverage ratio, and a stable recording with low signal interference can be available for high density arrays when they were better coupled with cells. These results indicate that the simulation increases the prospects for integrated active cellular electrodes and can be used to construct networks applied in the electrophysiological field.