电针胃经穴的大鼠血清对胃黏膜细胞EGFR阻断后c-myc的影响

The up-regulation of the ERK phosphorylation in gastric mucosal cell influenced by the serum derived from the rats treated with electroacupuncture at stomach meridian acupoints

目的:探讨电针胃经穴的大鼠血清对胃黏膜细胞EGFR阻断后c-myc基因的影响。方法:72只大鼠随机分为正常组、模型组、胃经组、胆经组、胃经+PD153035组和胆经+PD153035组,链霉蛋白酶消化法分离胃黏膜细胞,分别用表皮生长因子受体(EGFR)抑制剂PD153035和100 ml/L血清孵育胃黏膜细胞,RT-PCR法检测c-myc基因的表达水平。结果:胃经组和胆经组胃黏膜细胞Ec-myc基因的表达水平明显升高,与正常组、模型组比较均有非常显著性差异(P<0.01);胃经组胃黏膜细胞c-myc基因的表达水平升高最为明显,与胆经组比较有非常显著性差异(P<0.01);当用PD153035阻断EGFR后,胃经+PD153035组胃黏膜细胞c-myc基因的表达水平明显降低,与胃经组比较有非常显著性差异(P<0.01)。结论:电针胃经穴的大鼠血清能上调胃黏膜细胞c-myc基因的表达水平,并且存在经脉-脏腑的特异性联系。

Objective ：To study the Extracelluar Regulated protein Kinase （ERK） phosphorylation in gastric mucosal cell by the serum derived from the rats treated with electroacupuncture at stomach meridian acupoints. Methods：72 rats were randomly divided into Normal group, Model group, Stomach meridian group, Gallbladder meridian group, Stomach plus PD153035 group and Gallbladder plus PD153035 group,gastric mucosal cells were separated by using pronase digestion method,and incubated by PD153035 ,a EGFR inhibitor and 100ml/L serum in sequence, ERK phosphorylation in gastric mucosal cells was tested by Western blot. Results ： In both Stomach meridian group and Gallbladder meridian group, compared with the Normal group or Model group, the ERK phosphorylation in gastric mucosal cell were more reinforcement（P 〈 0.01 ）. Furthermore, the effects in Stomach meridian group were more obvious than those in Gallbladder meridian group（ P 〈 0. 01 ）. Compared with Stomach meridian group, the reduction of the ERK phosphorylation in Stomach plus PD153035 group was more significant（ P 〈0. 01 ）. Conclusions ：The serum derived from the rats treated with electroacupuneture at stomach meridian acupoints can activate the ERK phosphorylation in gastric mucosal cells,which also could he one proof for the TCM theory ＂Relative particularity between Meridian and Viscera＂.