LC-MS法及微生物效应法研究金银花提取物抗菌成分在SD大鼠体内药代动力学

Pharmacokinetics of antibacterial composition of Flos Lonicerae Japonicae extract in SD rat by LC-MS method and microbiological effect method

目的:金银花提取物抗菌成分在SD大鼠体内的药代动力学研究。方法:建立一种液质联用方法(LC-MS)定量测定大鼠血浆中的绿原酸。结果:此方法快速、灵敏、准确,并且与以抗菌活性为指标的微生物效应法有相关性。结论:此方法成功应用金银花主要抗菌成分药代动力学研究,为合理阐述金银花抗菌成分的体内药效物质基础提供一条新的思路。

Objective ： Pharmacokinetics of antibacterial composition of Flos Lonicerae Japonicae extract in SD rat was study. METHOD ： A LC-MS analysis method was developed to determine chlorogenic acid in plasma of SD rats. RESULT：The method was fast, sensitive and accurate. The result implied to the relevance with the method of microbiological effect. CONCLUSION ： The method was applied successfully for the pharmacokinetics of main antibacterial composition of Flos Lonicerae Japonicae extract. The paper would provides a new way to explain reasonably the active foundation of antibacterial composition of Flos Lonicerae Japonicae in vivo.