VEGF与膀胱尿路上皮癌微血管密度及预后的相关性研究

Correlation of VEGF expression with MVD and prognosis in human bladder urothelium cancer

目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)表达与膀胱尿路上皮癌微血管密度(microvessel density,MVD)及预后的关系。方法采用免疫组化的方法对50例原发性膀胱尿路上皮癌组织中VEGF进行染色,记数肿瘤内MVD,并观察VEGF的表达同MVD的关系。结果膀胱尿路上皮癌VEGF阳性表达率为62%(31/50),在正常膀胱黏膜组织中未见表达。VEGF阳性表达组MVD显著高于阴性表达组(P<0.05)。VEGF表达与肿瘤的分级分期明显相关(P<0.05),提示VEGF阳性表达者的预后较阴性者差。结论VEGF的异常表达是膀胱癌微血管形成能力增高的重要因素。VEGF表达与膀胱癌MVD增加及恶性进程和不良预后有关,是判断膀胱癌预后有价值的指标。

Objective To determine the relation of VEGF expression with MVD and prognosis in human bladder urothelium cancer. Methods The study included 50 patients of primary bladder urothelium cancer. We analyzed VEGF on paraffin embedded samples by immunohistochemical methods . Results The positive expression of VEGF was observed in 31 tumors（62%）. VEGF expression was significantly related with the MVD and infiltrative growth pattern（P〈0.05）. Patients with VEGF-positive bladder cancer had a poorer prognosis than those with VEGF-negative ones. Conclusions The abnormal expression of VEGF is the major element in increasing forming capability of the microvessel. The positive expression of VEGF is significantly related with MVD and tumor progression and poorer prognosis in bladder cancer. Quantification of VEGF may provide valuable markers for a poorer prognosis of patients.