慢性丙型肝炎病毒感染者血清中自身抗体检测结果分析

Analysis of test results of serum autoantibodies in patients with chronic hepatitis C

目的通过观察慢性丙型肝炎病毒(HCV)感染者血清中自身抗体的变化情况,探讨自身免疫反应在慢性丙型肝炎中的作用。方法用间接免疫荧光法对89例慢性HCV感染者血清测定抗核抗体(ANA)、可提取性核抗原抗体(ENA)、抗线粒体抗体(AMA)、抗平滑肌抗体(SMA),并根据荧光反应模式判定结果。结果慢性HCV感染者89例中有34例出现自身抗体,检出率为38.2%,高于慢性乙型肝炎自身抗体检出率13.8%(P<0.05),也高于健康对照组检出率2.1%(P<0.01)。其中慢性HCV感染者ENA阳性率为14.6%。结论HCV感染可触发机体自身免疫反应,自身免疫可能是HCV感染后组织损伤的重要因素。

Objective To investigate the role of autoimmune response in chronic hepatitis C by observing serum level change of autoantibodies in patients with chronic hepatitis C. Methods Antinuclear antibody （ANA） ,antibody against extractable nuclear antigen （ENA） ,antimitochondrial antibody （AMA） and antismooth muscle antibody （SMA） were detected by using indirect immunofluorescence assay in 89 patients with chronic hepatitis C. The test results were assessed according to fluorescence reaction patterns. Results The positive rate of autoantibodies was 38. 2% in chronic hepatitis C group, significantly higher than that in chronic hepatitis B group （13.8% ） （P〈0.05） and healthy control group （13.8%） （P〈0.01）. The positive rate anti-ENA antibody was 14.6% in the patients with chronic hepatitis C. Conclusion Autoimmune reaction can be triggered by HCV infection; and autoimmunity is an immporant factor for liver tissue injury in patients with chronic hepatitis C.