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蛋白转导域透膜作用的研究进展

Progress in Inchylosis of Protein Transduction Domain
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摘要 HIV-TAT蛋白转导域(Protein transduction domain,,PTD)是新近发现的一种在蛋白转导过程中能高效穿过生物膜的结构域,源自人类免疫缺陷病毒Tat蛋白的一段碱性氨基酸多肽,能与多肽、蛋白质及DNA等分子连接并跨膜导入绝大部分的组织细胞或透过血脑屏障,转导效率高且对细胞无损伤。TAT-PTD与细胞膜之间的电荷作用,吸附于膜表面,依赖脂筏介导的巨胞饮作用进入细胞。TAT融合蛋白系统是一种极有价值的运载工具,在基础医学研究和临床治疗方面有着广泛的应用前景。 HIV-TAT protein transduction domain （PTD）, a base peptide of tat protein discovered recently from human immunodeficiency virus type I （HIV-1）, can transfer protein across cell membrane in most tissues or across blood-brain barrier by binding with peptides, protein or DNA with high efficiency and no cytotoxicity. Protein transmembrane by TAT-PTD is associated with lipid-raft-mediated macropinocytosis by initial-ionic interaction. TAT fusion protein system is a very valuable delivery tool and widely used for the medical research and clinical application in diseases treatment.
作者 汤莹 史道华
机构地区 福建医科大学福总医学院药学科 南京军区福州总医院药学科
出处 《现代生物医学进展》 CAS 2009年第14期2756-2758,2762,共4页 Progress in Modern Biomedicine
基金 福建省自然科学基金项目(No.2008J0103) 南京军区医学科学技术研究"十一五"计划课题(06MA154)
关键词 蛋白转导域 人免疫缺陷病毒反式激活蛋白 融合蛋白传递 透膜作用 Protein transduction domain Human immunodeficiency virus trans-activator protein Fusion protein transfer Transmembrane
分类号 Q73 [生物学—分子生物学] Q819 [生物学—生物工程]
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参考文献28

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二级参考文献9

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现代生物医学进展
2009年 第14期

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