YS型小麦温敏雄性不育系A3314育性转换期间幼穗和叶片中物质含量的变化

Changes of material content in panicles and leaves of YS type thermo-sensitive male sterile wheat line A3314 during transfer from sterility to fertility

【目的】揭示YS型小麦温敏雄性不育系育性转换的有关生理生化机制。【方法】以YS型小麦温敏雄性不育系A3314为材料,在拔节至开花期给予日平均温度17.5℃(不育条件)和22.0℃(可育条件)处理,研究A3314不同发育阶段叶片和幼穗中脯氨酸、游离氨基酸、还原糖和可溶性糖含量的变化。【结果】在日平均温度22.0℃处理条件下,A3314叶片和幼穗中脯氨酸、游离氨基酸、还原糖和可溶性糖含量明显高于日平均温度17.5℃处理,尤其幼穗变化更为明显,且A3314各种物质含量与其原同型保持系TSP3314B的变化趋势基本一致。【结论】在可育条件下(日平均温度22.0℃),温度变化导致的YS型小麦温敏雄性不育系A3314的育性转换伴随着脯氨酸、游离氨基酸、还原糖、可溶性糖含量的变化,说明脯氨酸、游离氨基酸、还原糖、可溶性总糖的变化与该温敏不育系的育性密切相关。

【Objective】 The study was to reveal the related physiological biochemistry mechanism by which fertility was transformed YS wheat thermo-sensitive male-sterile line.【Method】 Experiments on physiological biochemistry study of the thermo-sensitive male-sterile wheat line A3314,which was bred by applying the technology of Chinese invention patent ZL00105488.0,were processed through the daily average temperature 17.5 ℃ and 22.0 ℃ at three different developmental stages（pollen mother cell of formation,pollen mother cell meiosis and heading date）during transfer from sterility to fertility. Contents of proline, amino acid, reducing sugar and soluble sugar in panicle and leaves of thermo-sensitive male sterile wheat line A3314 were analyzed. [~Result~ Contents of proline, amino acid, reducing sugar and soluble sugar in panicle and leaves of thermo-sensitive male sterile wheat line A3314 under the daily average temperature 22.0 ℃ condition were higher than under the daily average temperature 22.0 ℃ condition without panicle and leaves, especially the changes in panicle were the most obvious. The changes of each material content in A3314 under daily average temperature 22.0 ℃ process condition were basicly consistent with the change tendency of material content of the original same-type maintenance line TSP3314B. [Conclusion] During transfer from sterility to fertility on YS type thermo-sensitive male sterile wheat line A3314 under fertile condition,contents of proline,amino acid, reducing sugar and soluble sugar in panicle and leaves have significance changes,which explains the changes of each material content are closely correlated to fertility of this termo-sensitive male-sterile line.