间接免疫荧光法检测冠心病患者外周血单个核细胞PP65抗原

Detection of HCMV pp65 antigen in peripheral blood mononuclear cells of patients with CHD by indirect immunofluorescence assay

目的:了解冠心病患者外周血单个核细胞巨细胞病毒活动感染情况,并探讨动脉粥样硬化与HCMV感染的关系。方法:运用间接免疫荧光的方法,对29例冠心病患者、39例其他心血管病患者以及42例正常对照组外周血单个核细胞巨细胞病毒PP65抗原进行检测,并运用免疫散射比浊法对各组CRP进行测定。结果:29例冠心病患者中巨细胞病毒pp65抗原阳性者7例,占24.1%;与其他心血管患者中巨细胞病毒pp65抗原阳性率(5.1%)以及正常对照组巨细胞病毒pp65抗原阳性率(2.4%)相比明显升高,29例冠心病患者CRP水平(5.06±1.79mg/L)也明显高于其它两组(P<0.05)。结论:冠心病患者外周血单个核细胞巨细胞病毒活动感染可能在动脉粥样硬化的发展过程中有重要作用。

Objective：To detect human eytomegalovims（HCMV） phosphoprotein 65 （pp65） in peripheral blood mononuclear cells （ PBMCs ） of patients with enronary beart disease（CHD）, and to study the association between atherosclerosis and HCMV infection. Methods： HCMV #5 antigen in peripheral blood. leucocyte were detected by indirect immunofiuorescence assay in the group of 29 patients with coronary heart disease, and the group of 39 patiens with other heart disease, and 42 normal control, meanwhile the level of serum Creacfive protein （CRP） were detected with rate scat ter nephelometry in three groups Results： Positive rate of HCMV PP65 antigen in 29 patients with CHD was 24.1%, and the results was significantly higher than that in the group of 39 patiens with other heart disease（5.1% ）, and 42 normal control（2.4 % ）, （ P 〈 0.05 ）. and The CRP level significantly higher in the patients with CHD（ 5.064 ± 1.79 mg/L） than in the patients with other heart disease and normal control group. Conclusion： Active HCMV infection can occurs in patients with CHD, and this may play an important role in die process of atherogenesis.