摘要
了解细胞调亡和Bcl-2蛋白表达在慢性胃炎、胃溃疡、胃粘膜不典型增生、胃癌中的规律和可能作用。方法:采用原位末端标记法(TUNEL法)检测细胞凋亡,采用免疫级化检测Bcl-2蛋白的表达。结果:慢性活动性胃炎、胃溃疡、轻度不典型增生、重度不典型增生、早期胃癌和进展期胃癌的凋亡指数分别为16.8±12.3%、24.1±20.0%、19.3±16.4%、15.7±15.2%、10.1±9.1%和6.3±6.0%,进展期胃癌显著低于早期胃癌和不典型增生(P<0.05)。而Bcl-2蛋白阳性率分别为9.4%、27%.6%、52.9%、75.0%、83.3%和46.7%,早期胃癌显著高于进展期胃癌。在Lauren分型中,杨型和弥散型胃癌的细胞凋亡指数、Bcl-2蛋白的表达分别为8.3±7.2%、38.9%和5.1±4.9%、58.3%,两型比较,细胞调亡指数及Bcl-2蛋白表达均有显著差异(P<0.05)。结论:在胃粘膜不典型增生、早期胃癌到进展期胃癌的发展中,细胞凋亡逐渐受到抑制。胃癌的形成与Bcl-2的高表达有关,而且主要的作用阶段可能在癌变早期。胃癌的Lauren分型与细胞凋亡指数及BcL-2蛋白表达密切相关。
Objective: To clarify the Significance of apoptosis and expression of bcl - 2 in gastric pre - carcinoma and can-cer. Methods :TUNEL methal was used to deect apoptosis. Immunohistochemical analysis was used to detect the expressions ofbcl - 2 protein . Results :In the Patients of chronic active gastritis, gastric ulcer, minor dysplasia , serious dysplasia , carly gastric cancer and advanced gastric cancer, apeptotic indexes were respectively as follows: 16.8 ±12.3% 、 24. 1 ± 20.0% 、 19.3 ±16.4%、15.7 ± 15.2%、 10.1± 9.1% and 6.3 ± 6.0%, the expression rates of bcl - 2 were as follows: 9.4%, 27.6%,52.9%,75 .0%,83 .3% and 46.7%. Apoptotic indexes in advanced gastric cancer was significantly higher than that in early gastric cancer and dysplasia(P < O.05). The expression rates of bel - 2 in early gastric cancer was siedcandy higher than thatin advaned gastric caner. In Lauren classfication, apoptotic indexes and the expression rates of blc - 2 in intestinal and diffuse gastric carcinomas were respectively 8 .3 ± 7 .2%, 38.9% and 5. 1 ±4.9%, 58 .3%, There were significantly difference in spop-totic indexes and the expression of bcl- 2 between intestinal and diffuse gastric carcinomas . Conclusion : From dysplasia ,early gastric ereinomas to advanced the fromation of gastric careinomas are closely related to the high expression of bcl - 2 and it maybe the phenomenon in the eally stage gastric caner. Apoptotic indexes and the expression of bel - 2 are forly relatad to Laurenclassification.
出处
《广东医学》
CAS
CSCD
1998年第7期489-490,共2页
Guangdong Medical Journal
基金
广东省医药科学技术研究基金
关键词
胃癌
细胞凋亡
BCL-2
Gastric cancer Dysplasia Apoptosis bcl-2