比较使用不同方法采集鼻咽部分泌物快速检测流感病毒的效果

Comparative advantages of different methods of collecting nasopharyngeal secretions for rapid detection of influenza virus

目的比较使用鼻咽拭子法及鼻咽部细导管负压吸取法采集鼻咽部分泌物快速检测流感病毒的效果。方法分别采用鼻咽拭子法及鼻咽部细导管负压吸取法采集1042例急性呼吸道疾病患者鼻咽部分泌物作为实验样本，使用胶体金免疫层析（GICA）法试剂盒检测甲型及乙型流感病毒抗原，并做出评价。结果使用以上两种方法采集患者鼻咽部分泌物作为实验样本快速检测流感病毒，所得结果有较好的一致性，结果之间差异无统计学意义（P均〉0．05）。结论用鼻咽拭子法取材快速检测流感病毒是一种可靠方法，较之鼻咽部细导管负压吸取法更加快捷、方便。

Objective To compare the effectiveness of different methods of collecting nasopharyngeal secretions by nasopharynx swab and nasopharyngeal underpressure suction catheter for rapid detection of influenza virus. Methods Nasopharyngeal secretions as the experimental samples of 1042 patients with acute respiratory tract disea- ses were collected by nasopharynx swab and nasopharyngeal suction catheter, and gold immunochromatographic assay （GICA） kit was applied for the detection of influenza viruses. Results The use of the above two methods collecting nasopharyngeal secretions as samples for rapid detection of influenza virus would get the same results. The difference between the two methods had no statistical significance （P 〉 0.05 ）. Conclusions Nasopharynx swab is a reliable method for rapid detection of influenza virus, which is fast and convenient, compared with nasopharyngeal suction catheter.