高效毛细管电泳分析重组人红细胞生成素

Analysis of recombinant erythropoietin by capillary electrophoresis

目的：本实验旨在采用仪器分析的手段，测定重组人红细胞生成素（ｒＨｕＥＰＯ）的唾液酸化程度。方法：利用ｒＨｕＥＰＯ中唾液酸带负电荷的特性，通过高效毛细管电泳分离了唾液酸化程度不同的ｒＨｕＥＰＯ。结果：ｐＨ４．０的磷酸盐缓冲液可有效地涂渍毛细管壁，减少电渗流，提高分辨率。在优化的测定条件下共分出了６种糖基化形式。结论：建立的ＨＰＣＥ法能有效地反映ｒＨｕＥＰＯ的纯度。

OBJECTIVE: To analyze the levels of Neu5Ac by instrumental methods. METHODS: High performance capillary electrophoresis was applied to separate the glycoforms derived from different levels of Neu5Ac in rHuEPO. RESULTS: The phosphate buffer of pH 4.0 could deactivate the capillary wall effectively, so that the best resolution was obtained because of the reduced electroosmotic flow. CONCLUSIONS: The HPCE method established here could be employed to effectively detect the purity, microheterogeneity and in vivo bioactivity.