低氧诱导因子-1α在人脑胶质瘤中的表达及意义

The expression and significance of HIF-1α in human gliomas of different pathological grade

目的研究低氧诱导因子-1α(HIF-1α)。在不同病理级别人脑胶质瘤中的表达情况,探讨其与病理级别间的关系。方法收集67例人脑胶质瘤标本以及10例行内减压术切除的正常脑组织标本,采用免疫组织化学方法研究HIF-1α在不同病理级别胶质瘤及正常脑组织中的表达情况。结果HIF-1α阳性表达率在Ⅲ、Ⅳ胶质瘤中显著高于Ⅱ级胶质瘤,分别为72.7%、80.0%、36.0%,在正常脑组织中未见阳性表达,随着病理级别的增高,HIF-1α的表达阳性强度也相应增加(r=0.518)。结论HIF-1α在人脑胶质瘤中存在高度表达,其阳性表达率及表达阳性强度随着病理级别增加而上升。

Objective To investigate the expression of HIF-1α in human gliomas and the relationship between the ex- pression level and the pathologieal grade. Methods 67 surgical specimens were obtained from patients with gliomas of different pathological grading and 10 normal brain tissue were obtained from patients received internal decompression surgery because of craniocerebral trauma. The expression level of HIF-lct in gliomas and normal brain tissue was detected by immunohistochemistry. Rasults HIF-lct positive expression was observed in 36%grade Ⅱ gliomas,in 72.7% grade Ⅲ gliomas,in 80% grade Ⅳ glio- mas. No positive expression of both HIF-1α was observed in normal brain tissue. The positive exprssion rate of HIF-1α in grade Ⅲ and IV glioma was obviously increased compared with grade Ⅱ gliomas. In accordance with the increasing of glioma grades, the expressing intensity of HIF-1α was increased significantly too（ r=O. 518）. Conclusion HIF-1α was highly expressed in humangliomas, the positive expression rate and the expressing intensity was increased in accordance with the increasing of glioma grades.