阴阳平补中药对雄激素致不孕大鼠肝细胞膜胰岛素受体的影响

Effect of Kidney Yin-Yang Replenishing Chinese Herbs on the Binding Character of Insulin Receptor on Hepatocyte Membrane in Androgen Induced Sterility Rats

目的 :观察阴阳平补中药治疗前后雄激素致高胰岛素高雄激素不孕大鼠肝细胞膜胰岛素受体的变化。方法 :建立雄激素致高胰岛素高雄激素不孕大鼠模型 ,采用阴道涂片的方法观察阴阳平补中药对不孕大鼠的促排卵作用 ;采用放射配体结合分析方法检测肝细胞膜胰岛素受体的数目与亲和力。结果 :阴阳平补中药对雄激素致高胰岛素高雄激素不孕大鼠的促排卵率为 43 1 % ,而服药与未服药的不孕大鼠 ,其肝细胞膜胰岛素受体的数目及亲和力均与正常大鼠相似。结论 :阴阳平补中药对雄激素致高胰岛素高雄激素不孕大鼠的促排卵作用不是通过影响胰岛素受体的结合而实现。

Objective: To observe the effect of Kidney Yin Yang replenishing Chinese herbs on the binding character of insulin receptor (IR) on hepatocyte membrane from androgen induced sterility rats (ASR). Methods: The number and affinity of IR on hepatocyte membrane of ASR were determined with radioligand binding assay. Results: There was no significant difference of the number or affinity for both high and low affinity sites of IR between normal and ASR. Conclusion: The treatment with Kidney Yin Yang replenishing Chinese herbs had no effect on insulin binding although a relatively high rate of ovulation induction on ASR was observed.