川芎嗪、丹参对心肌成纤维细胞胶原合成和细胞增殖的影响

Effects of Tetramethylpyrazine and Radix Salviae Miltiorrhizae on Collagen Synthesis and Proliferation of Cardiac Fibroblasts

目的 :为了研究川芎嗪、丹参两味中药对体外培养心肌成纤维细胞 (fibroblasts,Fbs)的胶原合成与细胞增殖的影响。方法 :采用胶原酶和胰蛋白酶消化SD大鼠的心肌组织块 ,制备成纤维细胞的单细胞悬液 ,按不同药物及药物的不同浓度分组研究它们对成纤维细胞的胶原合成和增殖影响。结果 :中、高浓度的川芎嗪、丹参能有效地抑制成纤维细胞的胶原合成和增殖。低浓度的川芎嗪、丹参能对抗去甲肾上腺素对成纤维细胞的胶原合成与细胞增殖。结论 :川芎嗪、丹参能抑制成纤维细胞的胶原合成与细胞增殖。这些效应的机制可能与它们的钙离子拮抗有关。

Objective: To explore the effects of Tetramethylpyrazine (TMP) and Radix Salviae Miltiorrhizae (RSM) in collagen synthesis and proliferation of cardiac fibroblasts. Methods: Using collagenase and trypsase digested rat cardiac tissue assay to isolate cardiac fibroblasts (Fbs). Different dosage of TMP, RSM and norepinephrine (NE) were used to study their effects on the collagen synthesis and proliferation of cultured cardiac Fbs. Results: Compared with the control group, moderate or high dosage TMP and RSM could significantly inhibit the collagen synthesis and the proliferation of cultured cardiac Fbs. Moreover, low dose TMP(50mg/L) and low dose RSM(3g/L) could antagonize the collagen synthesis and the proliferation of cultured cardiac FB stimulated by NE (500μg/L). Conclusion: Both TMP and RSM could inhibit these processes. The mechanisms of these effect might be correlated to their Ca ++ antagonistic action.