cyclinD1、p16、cyclinA1在瘢痕疙瘩和增生性瘢痕中的表达及意义

Expression and significance of cyclinD1,p16 and cyclinA1 in keloids and hypertrophic scars

目的:观察瘢痕疙瘩和增生性瘢痕中成纤维细胞的cyclinD1、p16和cyclinA1的表达,探讨其表达的差异与两种不同类型瘢痕的形成之间的关联性。方法:实验组分为瘢痕疙瘩和增生性瘢痕两组,以正常皮肤组织作为对照组,通过SP免疫组化检测两实验组中cyclinD1、p16和cyclinA1的表达。结果:cyclinD1在瘢痕疙瘩的阳性表达率为91.7%,显著高于增生性瘢痕组的45.5%以及对照组(P<0.05);P16在增生性瘢痕的阳性表达率为81.8%,显著高于瘢痕疙瘩组的33.3%以及对照组(P<0.05);cyclinA1在瘢痕疙瘩的阳性表达率为75.0%,显著高于增生性瘢痕组的27.3%(P<0.05);cyclinD1和cyclinA1在增生性瘢痕组的表达率以及P16在瘢痕疙瘩组的表达率均高于正常皮肤组,但统计学分析其差异并不明显。结论:在瘢痕的形成过程中,cyclinD1和p16作为一对细胞周期的正负调控因子和cyclinA1作为正调控因子的表达失衡对瘢痕的不同转归是重要的参与机制。

Objective：To investigate the expression of cyclinD1,P16 and cyelinAl in the fibroblasts of keloids and hypertrophic scars, and explore the relationship between the genesis of two different kinds of scars. Methods：Normal skin tissues were used as control group,and the expression of cyclinDl,pl6 and eyclinA1 were screened in hypertrophic scars and keloids by immunohisto- chemical SP method. Results：In keloids,positive expression rate of eyclinD1 is 91.7%, that obviously higher than 45.5% in the hypertrophic scar group as well as the control group （P 〈 0.05） ;81.8% as the positive rate of p16 in hypertrophic scar group is significant higher than the rate of keloid group,which is 33.3% as well as the control group （P 〈 0.05） ;the rate of cyclinA1 in keloids （75.0%） is higher than hypertrophic scar group （27.3%） as well as the control group（P 〈 0.05）;cyclinD1 and cyclinA1 in hypertrophic scars, as well as the expression rate of P16 in the keloid group,are higher than that in the normal skin group,but the difference are not significant. Conclusion：In the progress of scar formation,the imbalance expressions of cyclinD1 and p16,as one pair of the positive and negative cell cycle regulatory factors and positive regulatory factor of cyclinA1, play important roles of the prognosis of scars.