毛细管区带电泳法测定复方马来酸依那普利片中两组分含量

Determination of Two Constituents in Compound Enalapril Maleate Tablets by Capillary Zone Electrophoresis

用毛细管区带电泳法同时测定复方马来酸依那普利片中两组分含量。以咖啡因为内标，２０ｍｍｏｌ／Ｌ硼砂—２０ｍｍｏｌ／Ｌ磷酸二氢钠（４９∶５１，ｐＨ８６）为运行缓冲液，在７ｍｉｎ内完成分离。马来酸依那普利和氢氯噻嗪的线性范围分别为８０～６４０μｇ／ｍＬ（ｒ＝０９９９９）和５０～４００μｇ／ｍＬ（ｒ＝０９９９３）。平均回收率分别为１０１０％和１０１１％，ＲＳＤ分别为１０％和１７％，ｎ＝５。

A simultaneous determination of two constituents in enalapril maleate compound tablets by capillary zone electrophoresis was reported.With caffeine as internal standard and 20 m mol/L borax — 20 m mol/L NaH2PO4(49∶51,pH 86)as running buffer,the two constituents were separated within 7 minutes.The linear ranges of enalapril maleate and hydrochlorothiazide were 80～640 μg/mL(r=09999) and 50～400 μg/mL(r=09993),respectively.The average recoveries(n=5) were 1010 % and 1011 %,RSD were 10 % and 17 %,respectively.