以体外侵袭力构建高转移膀胱癌细胞亚系

Establishing high metastatic bladder cancer subcell lines according to their in vitro in vasion ability

以穿透人工基底膜能力为依据筛选具有体外高侵袭能力的膀胱癌细胞，观察侵袭与转移的关系。用体外培养法收集扩增能侵袭穿透人工基底膜的细胞，对比筛选前后细胞体内外增殖和侵袭转移能力。结果显示：筛选后的子代细胞体外侵袭力均有明显的提高，其中有２株子代细胞裸鼠皮下接种后能形成肿瘤局部侵袭和淋巴结转移。表明侵袭对转移形成有重要影响，但完成转移还需要其它因素的参与。转移能力与细胞增殖有一定关系，但却分别受不同因素控制。

Using a specially constructed in vitro invasion chamber cells that penetrate the Matrigel were selected and cultured to establish offspring cell lines.The difference between offspring and parent cell lines in proliferation and metastasis were investigated.The results revealed (1)In vitro invasion ability of all offspring cell lines were enhanced significantly,Es3 and Ts3 cells had acquired ability of lymph node metastasis in nude mice but Bs3 could not form metastasis.This suggested that invasion is a very important property for metastasis formation but some other properties are also required (2) The proliferation rate of Es3 and Ts3 in nude mice was not different significantly comparing to their parental cells.This suggested that metastasis and tumor cell proliferation were controlled by different mechanisms.