胸腺内注射可溶性异基因主要组织相容性复合物抗原诱导皮肤移植特异性耐受

Induction of donor-specific tolerance to skin graft by intrathymic inoculation of allogeneic histocompatibility antigen from C57BL/6 mice

用３ｍｏｌ／ＬＫＣｌ从Ｃ５７ＢＬ／６小鼠脾细胞提取可溶性主要组织相容性复合物（ＭＨＣ）抗原，注射到ＢＡＬＢ／Ｃ受体鼠胸腺内，诱导了成年小鼠对该异基因小鼠皮肤移植物的耐受。除在胸腺注射当天及第３天给予抗Ｔ细胞单克隆抗体外，不使用免疫抑制剂。实验组移植皮肤平均存活时间（ＭＳＴ）为８３天，对照组ＭＳＴ为１１天。诱导耐受的小鼠对第３供体的移植皮肤仍正常排斥（ＭＳＴ为１２天）。单向混合淋巴细胞反应，耐受小鼠脾脏淋巴细胞对特异供体的脾细胞无反应，对第３供体的脾细胞反应正常，对丝裂原刺激的增殖反应正常。显示诱导的耐受是供体特异性的，无非特异性免疫抑制。

Intrathymic (IT) inoculation of allogeneic major histocompatibility complex (MHC) soluble antigen extracted from C57BL/6 inbred mice splenocytes using 3mol/L KCl was performed to induce donorspecific tolerance to skin allografts in adult BALB/C mice. The recipients(BALB/C) received antiT lymphocyte monoclonal antibodies (mAb) 8.4μg on 0 and 3rd day relative to IT injection of allogeneic MHC antigen without other immunosuppressant. The control group received antiT lymphocyte mAb 8.4μg but IT injection of normal saline (NS). Median survival time (MST) of transplanted skin allografts was 83 days in tolerant recipients while normally rejected 3rdparty CBA/J skin grafts (MST 12 days). In the control group, skin grafts’ MST was 11 days. Responder cells of tolerant recipients to C57BL/6 stimulators showed significantly less incorporation in oneway mixed lymphocyte reaction but in normal fashion to thirdparty CBA/J stimulators. The results suggested that unresponsiveness can be induced by IT inoculation of soluble allogeneic MHC antigen which was donor-specific. 