S108抑制大鼠肾移植排斥反应时细胞间粘附分子-1的表达

S108 inhibits the expression of cell adhesion molecule-1 (ICAM-1) during the rejection of renal allograft in rat

观测细胞间粘附分子－１（ＩＣＡＭ－１）在大鼠移植肾的表达，旨在探索Ｓ１０８抗排斥作用的机理以及对ＩＣＡＭ－１的表达有无抑制作用。共分５个实验组：即同品系移植组、不用药对照组、短期单用环孢素Ａ（ＣｓＡ）组、小剂量ＣｓＡ联合Ｓ１０８组及单用Ｓ１０８组。采用小鼠抗大鼠单克隆抗体（ＩＡ２９）测定移植肾内ＩＣＡＭ－１的表达，每一标本采用计算机图象分析定量测定。结果表明，ＩＣＡＭ－１在同品系移植肾肾小管周围的毛细血管内皮细胞、静脉和肾小管仅呈微弱阳性表达，不表达于肾间质组织。排斥时ＩＣＡＭ－１在移植肾血管内皮细胞、组织间质和肾小管上广泛表达，表达水平高低与移植肾排斥强弱有相关性，Ｓ１０８可降低ＩＣＡＭ－１抗原分子在移植肾的表达水平。

In order to explore the anti-rejection mechanism of S108 and its inhibition on the ICAM-1 expression, the expression of ICAM-1 in rat renal isografts and allografts was studied. The rats were divided into 5 experimental groups. The expression of ICAM-1 in the renal grafts was determined using mouse antirat monoclonal antibody (IA29). Quantitative measurement was performed in each samples using computer imaging analysis. The results showed that the expression of ICAM-1 presented faint positive in the capillary endothelial cells, veins around the tubules and the tubules in the same strain renal grafts. No ICAM-1 expression was found in the renal intestitial tissue. The extensive expression of ICAM-1 was found in the endothelial cells, intestitial tissue and tubules of the renal grafts during the rejection with the expression level relative to the severity of the rejection of the renal grafts. S108 could reduce the expression level of ICAM-1 in the renal grafts, so as to present antirejection reaction. 