安氟醚对缺血性预处理大鼠心肌抗氧化能力的影响

Effects of enflurane on antioxidant ability of ischemic preconditioned rat hearts in vitro

目的:了解安氟醚对缺血性预处理心肌抗氧化能力的影响。方法:SD大鼠96只,离体心脏Langendorff灌注,随机分为对照组,安氟醚组,缺血性预处理组和安氟醚加缺血性预处理组。各组均缺血25分钟,复灌30分钟造成心肌损伤,连续监测左室压和心率,分别在缺血前、缺血15分钟和复灌30分钟取心肌测超氧化物歧化酶(SOD)和丙二醛(MDA)。结果:复灌期实验组心肌舒缩功能的恢复显著优于对照组(P<0.05)。经缺血预处理两组心肌功能的恢复优于单纯安氟醚组(P<0.05)。实验各组SOD活性明显高于对照组,而MDA含量明显低于对照组(P<0.01)。安氟醚加缺血预处理组的SOD,MDA的变化又明显优于其他实验组。结论:安氟醚对缺血预处理心肌的抗氧化能力有明显的增强作用。

Objective:To investigate the effects of enflurane on antioxidant ability in ischemic preconditioned rat hearts in vitro. Method:Ninety-six SD rat hearts were perfused with Langendorff device. All hearts were randomly allocated to four groups:Control, enflurane group receiving 40 min of enflurane (1.5MAC),ischemic preconditioning group undergoing two 5-min ischemia and 10-min reperfusion, enflurane + ischemia group receiving enflurane (1.5MAC) for 10 min before ischemia preconditioning procedures. All groups underwent 25-min ischemia and 30-min reperfusion. Left ventricular pressure (LVP), heart rats (HR)and ischemic contracture pressure (ICP) were monitored. Myocardial superoxide dismutase(SOD)and malodialdehyde(MDA)were measured before ischemia, 15min following ischemia and 30 min following reperfusion. Result: Treated groups had better myocardial functional recovery of contractility compared to control groups,with preconditioning being better than enflurane (P<0.05). SOD levels in treated groups were significantly higher and MDA levels were significantly lower than those in control group (P<0.01). The improvement of SOD and MDA levels in enflurane+preconditioning group was better than in other treated groups (P<0.05). Conclusion:Enflurane can enhance the antioxidant ability of ischemic preconditioned rat hearts.