头状蓼提取物体外抗氧化活性研究

In Vitro Antioxidant Activity of Extracts from Polygonum alatum

目的:研究头状蓼提取物的体外抗氧化活性。方法:以脂溶性维生素E及二丁基羟基甲苯(BHT)作对照,采用清除二苯代苦味酰基(DPPH)自由基、清除[2,2′-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐](ABTS)自由基和铁离子(Fe3+)还原/抗氧化能力(FRAP)测定法检测头状蓼石油醚、乙酸乙酯、甲醇提取物的体外抗氧化活性。结果:与头状蓼石油醚、乙酸乙酯提取物比较,头状蓼甲醇提取物清除DPPH自由基、清除ABTS自由基及还原Fe3+的能力较强。结论:头状蓼甲醇提取物具有较好的体外抗氧化活性。3种方法中,DPPH方法和ABTS方法相关性最高。

OBJECTIVE： To study the in vitro antioxidant activity of the extracts from Polygonum alaturn .METHODS： 2, 2 - diphenyl - 1 - picrylhydrazyl assay（DPPH）, 2, 2＇ - amino - di（3 - ethyl - benzothiazoline sulphonic acid - 6） ammonium salt assay（ABTS） and ferric reducing antioxidant power assay（FRAP） were employed to analyze the antioxidant activity of extracts（ligarine extract, acetic ether extract and methanolic extract） from P.alatum with lipid- soluble vitamin and dibenzylatiooluene（BHT） as controls.RESULTS： Compared with ligarine extract and acetic ether extract, the methanol extract of P.alatum showed higher potency in scavenging DPPH radical and ABTS radical and reducing Fe^3＋ .CONCLUSION： The methanol extract of P.alatum showed high antioxidant activity in vitro. Among the 3 methods, the closest correlation was noted between DPPH assay and ABTS assay.