原因不明复发性流产与CD56CD16NK细胞的相关性研究

Correlational study on unexplained recurrent spontaneous abortion and CD56CD16 NK cells

目的:探讨原因不明复发性流产(URSA)与外周血和蜕膜组织中CD56+CD16-、CD56+CD16+NK细胞水平及CD56+CD16-/CD56+CD16+平衡的相关性。方法:选取30例早孕原因不明复发流产患者为试验组,20例正常早孕人流妇女为对照组,采集两组病例的蜕膜组织及外周血标本,分离淋巴细胞,用流式细胞技术检测各组标本中CD56+CD16-、CD56+CD16-NK细胞的数量,计算ED56+ED16-/CD56+ED16+平衡偏移。结果:两组外周血中CD56+CD16-细胞和CD56+CD16+NK细胞水平以及CD56+CD16-/CD56+CD16+比值差异均无统计学意义(P>0.05);两组蜕膜组织中,URSA组CD56+CD16+NK细胞水平升高,ED56+CD16-/ED56+CD16+比值降低,差异均有统计学意义(P<0.05),CD56+CD16-细胞与对照组比较差异无统计学意义(P>0.05);对照组蜕膜组织中CD56+CD16-NK细胞数及CD56+CD16-/CD56+CD16+比值均高于其外周血相应值,差异有统计学意义(P<0.05),CD56+CD16+NK细胞数在两组间差异无统计学意义(P>0.05);在URSA组中,蜕膜组织中的CD56+CD16-和CD56+CD16+NK细胞数均显著高于其外周血,差异均有统计学意义(P<0.05),CD56+CD16-/CD56+CD16+比值蜕膜组织中虽高于外周血,但无统计学意义(P>0.05)。结论:蜕膜组织中CD56+CD16+NK细胞水平升高以及由此引起的CD56+CD16-/CD56+CD16+比值的变化可能是原因不明复发性流产发生的一个主要因素。

Objective： To explore the correlation between unexplained recurrent spontaneous abortion （URSA） and the levels of CD56^＋CD16^- NK ceils, CD56^＋CD16^- NK cells, and the balance of CD56^＋CD16^-/CD56^＋CD16^- Methods： 30 women with URSA and 20 normal pregnant women were selected and regarded as experimental group and control group, respectively, their decidual tissues and pe- ripheral blood samples were collected and the lymphocytes were isolated, the amounts of CD56^＋CD16^- NK ceils and CD56^＋CD16^- NK ceils were detected by flow cytometry, the ratio of CD56^＋CD16^-/CD56^＋CD16^- were calculated. Results： There was no difference in the levels of CD56^＋CD16^-NK cells, CD56^＋CD16^- NK cells in peripheral blood, and the ratio of CD56^＋CD16^-/CD56^＋CD16^＋ between the two groups （P 〉0. 05）; in decidual tissues, the level of CD56^＋CD16^＋ NK cells increased and the ratio of CD56^＋CD16^-/CD56^＋CD16^-decreased in URSA group （ P 〈0. 05 ） , but there was no significant difference in the level of CD56^＋CD16^-NK cells between URSA group and control group （P 〉0. 05） ; in control group, the level of CD56^＋CD16^-NK cells and the ratio of CD56^＋CD16^-/CD56^＋CD16^- in decidual tissues were higher than those in peripheral blood （P 〈 0. 05 ） , but there was no significant difference in the level of CD56^＋CD16^- NK cells between URSA group and control group （ P 〉 0. 05） ; in URSA group, the levels of CD56^＋CD16^＋ NK cells and CD56^＋CD16^- NK cells in decidual tissues were higher than those in peripheral blood （ P 〈 0. 05 ）, but there was no significant difference in the ratio of CD56^＋CD16^-/CD56^＋CD16^- （P 〉 0. 05） . Conclusion： The ratio change of CD56^＋CD16^-/CD56^＋CD16^-induced by high level of CD56^＋CD16^-NK cells may be a main effect factor of URSA.