反胶束溶液和血红蛋白水溶液相平衡的研究

The Study of Hemoglobin-Reverse Micellar Solution Equilibria

研究了十六烷基三甲基溴化铵－正庚烷反胶束溶液和牛血红蛋白水溶液的相平衡，探讨了有机相助表面活性剂种类和浓度、水相ｐＨ值、水相离子种类和强度、表面活性剂浓度的影响，适宜条件下，牛血红蛋白的萃取率可达９５％以上。对实验结果从表面活性剂极性头与蛋白质分子间的相互作用和反胶团包溶蛋白质时的空间阻碍作用上进行了解释。

The optimum conditions of hemoglobin extraction by reversed micellar solution using surfactant CTAIB are studied, the pH value is 10.5-12.5, the concentration of KCI or NaCl is 0.1 mol/L, and of surfactant 0.02mol/L, the ratio of cosurfactant to surfactant is 0.3 The 95 percent of hemoglobin in aquous phase is transfered into reversed micellar solution with one extraction-stripling stage.