浙江有尾两栖动物分类与演化的RAPD研究

Taxonomic and Evolutionary Studies Using RAPD on Zhejiang Tailed Amphibians

选用20个随机短引物（10nt）和6个随机长引物（≥20nt）对来自3个科5个属7个种的有尾两栖动物进行RAPD分析，12个引物得到清晰的DNA扩增图谱，共计207条多态性片段．所得数据利用计算机软件进行聚类分析，建立系统树．结果表明，舟山小鲵与中国小鲵的遗传距离最近，大鲵与其他种之间的遗传距离最远．

Twenty short arbitrary primers (10 nt) and six long ones (20 nt) were used in RAPD analysis for Zhejiang tailed amphibians of seven species. Twelve primers could generate clear amplified patterns. Two hundred and seven polymorphic bands were obtained.UPGMA method was used to construct phylogenetic tree by computer software. The result showed that Hynobius chinensis and Hynobius sp. in Zhoushan had the smallest genetic distance, and Andrias davidianus was the most different from others.