灯盏细辛预处理脊髓缺血再灌注损伤后动作诱发电位和神经功能的变化

Changes of MEP and Neurologic Function after Erigeron beviscapus Preconditioning on Spinal Cord Ischemia-reperfusion Injury in Rabbits

目的观察灯盏细辛(EBHM)预处理对家兔脊髓缺血再灌注损伤后动作诱发电位(MEP)和神经功能的变化,为临床应用灯盏细辛治疗脊髓缺血再灌注损伤提供试验依据。方法24只家兔随机分为4组。假手术组(A组)只暴露但不夹闭腹主动脉;空白对照组(B组)暴露腹主动脉并夹闭40min后再灌注;甲基强的松龙对照组(C组)夹闭前30min经耳缘静脉静滴甲基强的松龙30mg/kg,术后连用2d。药物组(D组)夹闭前30min经耳缘静脉静滴灯盏细辛50mg/kg,术后连用2d。于夹闭前、夹闭后、再灌注后各时间点分别测量动作诱发电位,并于术后每天观察神经功能,共观察7d。结果在缺血再灌注后检测的各时段,各组动作诱发电位的潜伏期和波幅有规律性变化,C、D组与B组比较有显著性差异(P<0.05)。7d后神经功能观察各组间均有显著性差异(P<0.05)。结论MEP监测可以反映脊髓缺血再灌注损伤早期变化并能判断预后。灯盏细辛预处理对兔脊髓缺血再灌注损伤有保护作用。

Objective To observe the changes of MEP（motor evoked pontential） and neurologic function after Erigeron beviscapus （EBHM） preconditioning on spinal cord ischemia-reperfusion injury in rabbits, and provide substructural experimental theory. Methods Twenty-four domestic rabbits were randomly divided into four groups with six rabbits each. In sham group （group A） only the abdominal aorta was exposed. In the blank group （group B） , the abdominal aorta was occluded 40 minute followed by reperfusion. In the methylprednisolone group （group C） , it was occluded 30 minute before the abdominal, methylprednisolone （30 mg/ kg） was injected into the rabbit by vein, which was used for two days after the surgeon. In the EBHM group （group D）, EBHM（50 mg/kg） was injected into the rabbit by vein, the following procedure was the same as group C. MEP was measured in each observed point and neurologie function was observed everyday for 7days. Results At every observed point after SCI, the MEP changed regularly, and compared with group B, there was significant difference in groups C and D（P〈0.05）. 7 days later, the neurologic function changed significantly in each group （P〈0.05）. Conclusion MEP monitoring can reflect the early change on spinal cord ischemia reperfusion injury. EBHM preconditioning can protect the rabbit from spinal cord ischemia reperfusion injury.