丙戊酸对神经干细胞修复损伤脊髓功能的影响

Effects of Valproic Acid on Function Recovery of Injuried Spinal Cord with Transplanted Neural Stem Cells in Adult Rats

目的观察丙戊酸(VPA)对神经干细胞(NSCs)移植修复成鼠损伤脊髓功能的影响。方法SD大鼠96只,随机分为单纯损伤组、VPA治疗组、NSCs治疗组和NSCs+VPA治疗组。在T10节段造成半切脊髓损伤,于损伤后第2、4、8周进行BBB评分和神经诱发电位检测,在8周时用核黄和DIL进行示踪检查。结果NSCs+VPA治疗组核黄和DIL标记的神经元数量多于其他3组;NSCs+VPA治疗组BBB评分和诱发电位各项指标均优于其余3组,其中以运动诱发电位峰潜伏期的优化最明显。结论VPA能促进NSCs移植对成鼠损伤脊髓功能的修复作用。

Objective To observe the effects of valproic acid （VPA） on function recovery of injuried spinal cord with transplanted neural stem cell in adult rats. Methods 96 SD rats were divided into 4 groups randomly： the injury group, VPA group, NSCs group and NSCs＋VPA group. All rats were hemi-sected at T10 level. The rats in VPA group were injected with VPA 300 mg/kg·d introperitoneally twice a day. Those in NSCs group were transplanted with absorbable gelatin sponge absorbing the identified NSCs. Those in NSCs＋VPA group were dealed the same as those in NSCs group, and injected with VPA 300 mg/kg·d introper-itoneally twice a day. They were assessed with Basso-Beattie-Bresnahan（BBB） scale and electrophysiology examination in 2nd, 4th and 8th week. The nuclear yellow retrograde tracing and DIL anterograde tracing were performed in 8th week. Results The number of neurons traced with DIL and nuclear yellow of NSCs＋VPA group were more than that of other groups. The BBB scores and indexes of electrophysiology examination of NSCs＋VPA group improved more than other groups, especially the motor evoked potentials. Conclusion VPA promotes neural stem cells transplant to repair the function of injuried spinal cord in adlut rats.