单酶法快速测定发酵液中的L-乳酸

Single Enzyme Method for Rapidly Determining LLactic Acid in Fermentation Liquid

介绍一种简便、快速测定发酵液中Ｌ－乳酸的新方法．采用不依赖ＮＡＤ的Ｌ－乳酸脱氢酶（Ｌ－ＬＤＨ），以菲咯啉铁复合物（Ｏ－ｐｈｅｎａｔｈｒｏｌｉｎｅ－Ｆｅ３＋）作为其电子受体，吸收波长５１０ｎｍ，在７５１分光光度计上直接测定发酵液中的Ｌ－乳酸．

A new handy method is presented for rapidly determining Llactic acid in fermentation liquid. By adopting NADindependent Llactic acid dehydrogenase, and also Fe3+ phenanthnoline as electron acceptor, Llactic acid in fermentation liquid can be directly determined in 751 spectrophotometer, with an absorption wavelength at 510 nm.