头颈鳞癌信号转导与转录活化因子3的表达对顺铂敏感性的影响

Influence of STAT3 expression on cisplatin sensitivity in head and neck squamous cell carcinoma

目的明确STAT3在头颈鳞癌中的表达,探讨STAT3表达水平对头颈鳞癌顺铂敏感性的影响。方法用肿瘤原代细胞胶原凝胶体包埋化疗药物敏感性检测(collagen gel droplet embedded culture-drug sensitivity test,CD-DST)技术检测28例头颈鳞癌对顺铂的敏感性,免疫化学染色检测STAT3在头颈鳞癌、正常鳞状上皮和舌鳞癌细胞株Tb 3.1中的表达。Tb 3.1细胞分别经α氰基-(3,4羟基)N-苄苯乙烯胺(AG490)、二甲基亚砜(DMSO)处理后,CD-DST检测AG490处理组与DMSO处理组的顺铂敏感性。结果STAT3在头颈鳞癌组织中的表达水平高于正常鳞状上皮组织(χ2=13.137,P<0.005);头颈鳞癌顺铂耐药组STAT3表达水平高于顺铂敏感组(χ2=9.616,P=0.008);AG490处理组和DMSO处理组的顺铂敏感性差异具有统计学意义,(8.9±1.5)%vs(21.3±2.5)%(P=0.000)。结论头颈鳞癌STAT3的表达水平与顺铂敏感性呈负相关;利用AG490阻断舌鳞癌细胞株Tb 3.1中STAT3信号通路可提高其对顺铂的敏感性。

Objective To explicit the expression level of STAT3 in head and neck squamous cell carcinoma,and explore the influence of STAT3 expression on cisplatin sensitivity. Methods The cisplatin sensitivity in 28 cases of head and neck squamous cell carcinoma were detected by collagen gel droplet embedded culture-drug sensitivity test system（CD-DST）. The expression levels of STAT3 in head and neck squamous cell carcinoma, normal squamous epitheliums and Tb 3. 1 ce]ls were detected by immunohistochemistry. After Tb 3. 2 ceils were treated by AG490 or DMS（）, the cisplatin sensitivity in the AG490 treatment group and the DMSO treatment group was detected by CD- DST. Results The expression level of STAT3 protein was elevated in head and neck squamous cell carcinoma samples as compared with that of normal squamous epithelium（x2 = 13. 137, 12 d0. 005）. The expression level of STAT3 in the cisplatin resistant group was higher than that of the cisplatin sensitive group（x2= 9. 616, P = 0. 008）. There was significant difference in cisplatin sensitivity between the AG490 treatment group and the DMSO treatment group, （8.9±1.5）% vs （21.3±2.5）% （ P =0.000）. Conclusion There was a negative correlation between cisplatin sensitivity and STAT3 expression levels. The cispaltin sensitivity in the Tb3. 1 cells can be improved after STAT3 signaling is blocked by AG490.