苜蓿总黄酮对小鼠脂类代谢及氧自由基的影响

Impact of total flavone of alfalfa on the lipid metabolism and oxygen-derived free radicals in mice

为了明确观察苜蓿总黄酮(TFA)对小鼠脂类代谢和氧自由基的影响,给5周龄的小白鼠灌胃TFA,连续1周后,采用生化方法测定小鼠全血中甘油三酯、低密度脂蛋白胆固醇和高密度脂蛋白胆固醇的含量,并且检测小鼠肝脏和肾脏组织中过氧化物歧化酶的活性和丙二醛的含量。结果显示:灌胃TFA的小鼠全血中甘油三酯和低密度脂蛋白胆固醇的含量明显降低(P<0.05),而高密度脂蛋白胆固醇含量升高。小鼠的肝脏和肾脏组织匀浆中过氧化物歧化酶的活性显著升高(P<0.05),丙二醛的含量降低。因此得出结论:TFA具有降血脂、降低低密度脂蛋白胆固醇、预防和减轻动脉硬化的作用;同时也具有抑制氧自由基损伤防止脂质过氧化的作用。

The impact of total flavone of alfalfa （TFA） on the lipid metabohsm and oxygen-derived tree radicals in mice studied. After the mice were drenched with TFA for seven days, the contents of triglyceride （TG）, low density lipoprotein （LDL） and high density lipoprotein （HDL） in blood as well as the activities of superoxide dismutase （SOD） and malonaldehyde （MDA） in liver and ren were determined by biochemical methods. In TFA group, the contents of TG and LDL in blood and MDA in tissues were obviously reduced（P〈0.05）, but HDL-C content was high, and the activities of SOD in tissues were significantly increased（P〈0.05）. So it could be concluded that TFA could perform the function of reducing TG and LDL as well as preventing angiosclerosis, and at the same time, it could obviously inhibit the damage from oxygen-derived free radicals and prevent lipid peroxidation.