一个新的杆状病毒凋亡抑制基因的克隆及鉴定

ISOLATION AND CHARACTERIZATION OF AN APOPTOTIC SUPPRESSOR GENE FROM SPODOPTERA LITTORALIS NUCLEOPOLYHEDROVIRUS

缺失功能性ｐ３５基因的苜蓿尺蠖核形多角体病毒（ＡｃＮＰＶ）突变体（ｖΔＰ３５Ｋ／ｐｏｌ＋）感染草地夜蛾细胞（Ｓｆ９和Ｓｆ２１）［１］及海灰翅夜蛾细胞（ＳＬ２）［２］时，引起细胞凋亡，从而使病毒的感染流产．我们发现海灰翅夜蛾核形多角体病毒（ＳｌＮＰＶ）...

The Spodoptera littoralis nucleopolyhedrovirus was able to suppress apoptosis triggered by an AcNPV p35 null mutant. To identify the putative apoptotic suppressor gene of SlNPV, overlapping cosmid clones representing the entire SlNPV genome, were individually cotransfected along with genomic DNA of an AcNPV p35 null mutant. Using this complementation assay we identified a novel SlNPV gene, inhibitor of apoptosis, Sl p49. The Sl p49 sequence predicted a 49 KD polypeptide with about 48.8% identity to p35 of AcNPV. Recombinant AcNPVs, deficient in p35 and bearing the Sl p49 gene, showed successful productive infection in Sf9 cells indicating that Sl p49 is a functional homolog of p35.