依赖PQQ的甲醇脱氢酶与甘氨酸作用后的进程曲线

THE PROGRESS CURVE OF PQQ DEPENDING METHANOL DEHYDROGENASE AFFECTED BY GLYCINE

从革蓝氏阴性甲基营养菌Ｎｏ．２细胞提取的甲醇脱氢酶（ＭＤＨ）是一种氧化还原酶，已证明其辅基是新辅基ＰＱＱ（吡咯喹啉醌），笔者在研究ＭＤＨ及其辅基的理化性质过程中，发现甘氨酸（ｇｌｙ）对ＭＤＨ影响极为特殊，随后，以此为出发点得到的试验数据，对解释目前国...

In the assay of Methanol Dehydrogenase (MDH) discussed in this article,NH + 4 stimulates the activity of MDH (by about 100%),furthermore,it leads to the oxazole formation from enzyme prosthetic group PQQ and glycine.Oxazole not only blocks the electron transport and decreases the enzyme activity,but oxidizes reduced dichlorophenolindophenol (DCPIP) in the reaction mixture,at last results in the bell shaped progress curve.