病原真菌细胞壁对棉花β-1,3-葡聚糖酶的诱导

Induction of β-1, 3- Glucanase in Cotton by the Hydrolysate of Cell Wall from Fusarium Moniliforme

从引起棉花红腐病的病原真菌——串珠镰孢菌（Ｆｕｓａｒｉｕｍｍｏｎｉｌｉｆｏｒｍｅ）的菌丝中制备了细胞壁水解产物，测定了其对棉花叶片和棉花组培细胞的β－１，３－葡聚糖酶的诱导能力．结果表明，串珠镰孢菌菌丝细胞壁水解产物可以诱导棉花细胞的β－１，３－葡聚糖酶的活性增加．诱导过程需要钙离子．用水杨酸预先作用棉花组培细胞可以提高细胞对病原菌菌丝细胞壁水解产物的诱导敏感性，使β－１，３－葡聚糖酶和另一类与植物抗病反应有关的酶（过氧化物酶）达到最大活性的时间提前．

β 1, 3 glucanase plays an important role in defense reaction of plants. The hydrolysate of cell walls was prepared from the hyphae of fusarium moniliforme inducing moniformae in cotton seeding. The induction activities of the hydrolysate to β 1, 3 glucanase were detected in cotton leaves and cultured cells. The induction of β 1,3 glucanase and β 1, 3 glucanase was studied in pretreatment cultured cells by salicylic acid. The results show that the hydrolysate of cell wall from fusarium moniliforme induced β 1,3 glucanase and that calcium was needed during the induction. The pretreatment with salicylic acid promoted the response of enzymes POD and β 1, 3 glucanase in cotton cells. The induced accumulation of the emymes were accelerated by salicylic acid.