摘要
肺动脉收缩和重塑在肉鸡AS发生过程中起着重要作用.本实验经采用右心导管法研究发现AS患鸡PASP、PADP和mPAP极显著高于对照组(P<0.01).同时,采用焦锑酸钾沉淀法、电镜酶细胞化学法研究AS患鸡肺脏组织Ca2+和Ca2+-ATPase活性变化,发现AS患鸡肺脏组织中钙沉积量显著增多,且Ca2+-ATPase的电子密度颗粒显著减少或缺失.表明AS患鸡具有明显的肺动脉高压,其可能与肺脏组织,特别是肺动脉平滑肌细胞钙处理能力异常导致的钙离子浓度升高和肌浆网Ca2+-ATPase酶活性降低有关.
Pulmonary artery constriction and remodeling is a vital factor to the incidence of AS in broilers. The purpose of this experiment was to measure the pulmonary artery pressure and investigate the precise ultrastructural location of Ca2+ and Ca2+-ATPase in pulmonary tissure of chickens with ascites syndrome induced by low ambient temperature. The results showed that the pulmonary artery systolic pres- sure (PASP), the pulmonary artery diatolic pressure (PADP) and mean pulmonary artery pressure (mPAP) of ascitic broilers were significantly higher than that of control broilers. The increased calcium deposits were observed in the pulmonary tissure of aseitic broilers. The Ca2+-ATPase reactive grains were rarely observed in the ascitic broilers. The data suggested that there was obvious pulmonary hypertension in ascitic broilers. The overload of intraeellular calcium and inhibition of Ca2+-ATPase might be the important factors.
出处
《河北北方学院学报(自然科学版)》
2009年第4期39-42,共4页
Journal of Hebei North University:Natural Science Edition
基金
国家自然科学基金项目(30371063)
关键词
肉鸡
腹水综合征
肺脏组织
CA2+
Ca2+-ATPase
焦锑酸钾沉淀法
电镜酶细胞化学法
broiler
ascites syndrome
pulmonary tissue
Ca2+
Ca2+-ATPase
the potassium pyroan-timonate cytochemical method
the electron microscopic enzyme histochemistry