摘要
HARDY(HRD)是AP2/ERF-like家族转录因子,它的过量表达可以增强水稻的抗旱性、提高水稻的水分利用率。本研究从拟南芥中克隆了HRD转录因子基因,利用DNA重组技术,构建了HRD原核表达载体PET-HRD,并将PET-HRD重组质粒转化E.coli BL21(DE3),经lmmol/LIPTG诱导其蛋白质表达,获得了高表达量的分子量约为42kD的HRD融合蛋白。本研究结果为进一步制备HRD转录因子的抗体,并利用该抗体来分析和克隆不同物种的HRD基因奠定了基础。
In this study, we cloned the HRD gene from Arabidopsis thaliana（Columbia） genome by PCR method and constructed PET-HRD prokaryotic expression vector. The PET-HRD construction was transferred into E. coli BL21 （DE3）. After being induced by 1 mmol/L IPTG,the high level expression of the recombinant HRD protein was obtained and the molecular weight protein is 42kD. The results of this study has laid a foundation for the further preparation of HRD transcription factor antibodies and use of the antibody to analyze and clone HRD genes of different species.
出处
《石河子大学学报（自然科学版）》
CAS
2009年第4期397-400,共4页
Journal of Shihezi University(Natural Science)
基金
国家转基因专项(2008ZX08005-004)
新疆兵团博士基金(2006jc07)
关键词
HRD
拟南芥
转录因子
原核表达
HRD
Arabidopsis thaliana
transcription factor
prokaryotic expression
