口腔脱落细胞乙醇现场固定法微核试验的研究

Study of Micronucleus Test of Ethanol Field-fixed Buccal Mucosal Cells

目的建立乙醇现场固定口腔脱落细胞进行微核试验的方法。方法利用无水乙醇现场固定口腔脱落细胞,保存0～30h后进行微核试验与常规口腔脱落细胞微核试验、外周血淋巴细胞微核试验进行比较。结果乙醇现场固定法的口腔脱落细胞在4、8、24h后的微核率与常规口腔脱落细胞微核率和外周淋巴细胞法微核率(Oh)差异无统计学意义(P>0.05),而常规法口腔脱落细胞在保存4h后则出现微核率下降,与外周血培养法的微核率差异有统计学意义(P<0.05)。结论无水乙醇现场固定口腔脱落细胞能够有效延长保存时间,微核试验结果稳定,适合用于现场采样。

[ Objective] To estabhsh the micronucleus test of methanol fixed buccal mucosal cells in the field. [ Methods] Using the ethanol field-fixed buccal mucosal cell to do micronucleus test in 0- 30 hours, then compared with regular buccal mucosal micronucleus test and peripheral blood lymphocytes micronuclesus test in terms of the micronucleus incidence rate. [ Results ] The micronucleus incidence had no significant difference between the ethanol field - fixed buccal mucosal group in 4, 8, 24 hours and the peripheral blood lymphocytes group （ P 〉 0.05 ）. The mieronucleus incidence declined in the regular buccal mucosal group after 4 hours of preservation, compared with the ethanol field-fixed buccal mucosal cell group, there was significant difference （ P 〈 0.05 ）. [ Con- dusion ] The ethanol field - fixed buecal mucosal cells can extend the preservation of cells effectively, and its results of micronucleus test is stable, suitable for field sampling.