新选优良玉米自交系SSR遗传多样性分析

Genetic Diversity Analysis Among New Maize Inbred Lines Revealed by SSR

利用SSR标记技术研究了59份新选玉米自交系的遗传多样性,用58对SSR引物共检测出681个等位基因变异,每对引物检测到等位基因2～25个,平均为11.7个。聚类结果表明,大部分玉米自交系聚到4大常见类群,其他少部分材料形成单独的类群。所有材料中4大类群的占78%,其中Reid类群约占51%,且在相似系数0.727处又分为2个亚类,说明这部分材料亲缘关系较近,但也有一定的遗传差异;其他类群约占22%,说明这些材料与其他材料之间遗传差异较大。从系谱的亲缘关系分析,部分已知自交系其SSR聚类结果与系谱追踪结果有较好的一致性。

SSR markers （Simple Sequence Repeat） were used to detect genetic diversities among 59 maize inbred lines. 58 SSR primers screened were produced 681 polymorphic amplified fragments. The average number of alleles per SSR locus was 11.7 ranging from 2 to 25. The clustering results showed some inbred lines cluctered to four familiar groups occupied 78 percent, of which belonged to Reid group occupied 51 percent which were divided into two sub-groups. The pedigree among the inbred lines not only were kindred but also had inherited diversity. The other inbred lines belonged to other groups which occupied 22 percent. It meant there were biggish diversity between these groups and others. The pedigree of some known inbred lines was analyzed. The result of clustering by SSR were well consisted with family tree.