期刊文献+

鸦胆子乙醇提取物对血小板源性生长因子受体介导细胞迁移的抑制作用 被引量:3

Inhibitory effect of ethanol extract from Java Brucea Fruit on cell migration mediated by platelet-derived growth factor receptor β
下载PDF
导出
摘要 目的:观察鸦胆子乙醇提取物对血小板源性生长因子受体β(PDGFRβ)介导细胞迁移的影响,阐明其起作用的有效成分。方法:用脂质体介导的细胞转染技术将人PDGFRβ表达载体转染体外培养的不表达PDGFRβ的PAE细胞,G418抗性筛选建立稳定转染的细胞株,采用RT-PCR法检测PDGFRβ的表达;用细胞损伤修复实验观察细胞损伤24 h完全修复所需的血小板源性生长因子BB(PDGFBB)最低浓度;划痕实验观察加入不同浓度鸦胆子乙醇提取物24 h后,计算细胞迁移的抑制率及鸦胆子发挥抑制作用的主要成分溶于乙醇溶液的浓度。结果:RT-PCR检测转染组细胞表达PDGFRβ;细胞损伤修复实验检测细胞损伤24 h完全修复所需的血小板源性生长因子BB(PDGFBB)最低浓度为10μg.L-1;划痕实验检测随着鸦胆子乙醇提取物(70%乙醇溶液)浓度的增加,对细胞迁移的抑制率增加(P<0.05),呈剂量效应关系;50%乙醇提取物和25%乙醇提取物(浓度≤10μg.L-1)对细胞迁移抑制不明显,鸦胆子乙醇提取物浓度>10μg.L-1主要导致细胞死亡。结论:鸦胆子抗肿瘤作用机制可能是通过抑制PDGFRβ介导的细胞迁移来实现的,其发挥作用的主要成分更易溶于70%的乙醇溶液中。 Objective To study the effects of ethanol extract from Java Brucea Fruit on platelet-derived growth factor receptor β(PDGFRβ) mediated cell migration and to obtain the valuable messages on the characteristics of its active ingredient.Methods PAE cells was transfected with the vector expressing human PDGFRβ with Transfectom 2000;After screening by G418 resisitance,RT-PCR was used to monitor the expression of PDGFRβ in the cells;Wound healing of the cells was used to examine the lowest consistency of PDGFBB and inhibitory effect of ethanol extract of Java Brucea Fruit on cell migration after restoring 24 h.Results Human PDGFRβ was stably expressed in PAE cells transfected with the expressing vector.The lowest consistency of exogenous PDGFBB which promoted PDGFRβ mediated cell migration was 10 μg·L^-10% ethanol extract of Java Brucea Fruit which strongly inhibited PDGFRβ mediated cell migration was dose-dependent(P〈0.05),50% and 25% ethanol extract from Java Brucea Fruit(≤10 μg·L^-1had the weaker inhibitory effect,ethanol extract from Java Brucea Fruit(〉10 μg·L^-1mainly caused the death of the cell.Conclusion Ethanol extract of Java Brucea Fruit has a strongly inhibitory effect on the PDGFRβ mediated cell migration which could play a major role in its effects against metastasis of malignant tumor,the active ingredients of it could be more dissolvable in the 70% ethanol.
出处 《吉林大学学报(医学版)》 CAS CSCD 北大核心 2009年第4期608-611,F0002,共5页 Journal of Jilin University:Medicine Edition
基金 国家863重大专项资助课题(2004AA205020) 吉林大学留学回国科研启动基金资助课题[科技字(419)号]
关键词 鸦胆子乙醇提取物 恶性肿瘤 血小板源性生长因子受体 细胞迁移 Java Brucea Fruit extract malignant tumor platelet-derived growth factor receptor cell migration
  • 相关文献

参考文献7

二级参考文献20

  • 1孙毓庆 王立群 赵立平 等.鸦胆子抗肿瘤的研究:脱酯鸦胆子油的色谱-质谱分析[J].沈阳药学院学报,1981,12(14):13-23.
  • 2Anderson M M, O'Neill M J, Phillipson J D, et al. In Vitro Cytotoxicity of a Series of Quassinoids from Brucea Javanica Fruits Against KB Cells. Planta Med, 1991, 57(1): 62.
  • 3Xu H X, Liu Y H, Cheng X W. A Study on the Available Anticancer Composition of Brucea Javanica.中草药, 1986, 11(12):529.
  • 4Ohnishi S, Fukamiya N, Okano M, et al. Bruceosides D, E, and F, Three New Cytotoxic Quassinoid Glucosides from Brucea Javanica. J Nat Prod, 1995, 58 (7) : 1032.
  • 5Xuan Y B, Yasuda S, Shimada K, et al. Growth Inhibition of the Emulsion from to Brucea Javanica Cultured Human Carcinoma Cells. Gan To Kagaku Ryoho, 1994, 21(14) : 2421.
  • 6Fukamiya N, Okano M, Miyamoto M, et al. Antitumor Agents,127. Bruceoside C, A New Cytotoxic Quassinoid Glucoside, and Related Compounds from Brucea Javanica. J Nat Prod, 1992, 55(4): 468.
  • 7Lu l B, Shu S Y, Cai I Q. Experimental Study on Effect of Brucea Javanica Oil Emulsion on Rabbit Intracranial Pressure. 中国中西医结合杂志, 1994, 14(10): 610.
  • 8Wang Z Q. Combined Therapy of Brain Metastasis in Lung Cancer. 中国中西医结合杂志, 1992, 12(10) : 609.
  • 9Keer J F R, Winterford C M, Harman B V. Apoptosis, it's Significance in Carcinoma and Carcinoma Therapy. Carcinoma,1994, 73: 2013.
  • 10Shigekazu, Nagata, Golstein P. The Fas Death Factor. Science,1995, 267:1449.

共引文献90

同被引文献20

引证文献3

二级引证文献14

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部