山葡萄多酚对酒精慢性中毒大鼠氧化损伤的保护作用

Protective effects of polyphenols of vitis amurensis Rupr on rat oxidative damage caused by chronic alcohol intakes

目的:研究山葡萄多酚(PVAR)对酒精慢性中毒大鼠肝脏氧化损伤的保护作用,阐明PVAR防治酒精性肝病(ALD)的理论基础。方法:Wistar雄性大鼠40只,按体重区间随机分4组,每组10只:对照组,酒精组(33%酒精10 mL.kg-1灌胃),低、高剂量PVAR组(33%酒精10 mL.kg-1灌胃前,灌胃200和400 mg.kg-1PVAR)。连续实验8周后,检测大鼠肝脏组织丙二醛(MDA)、活性氧(ROS)、谷胱甘肽(GSH)含量、超氧化物歧化酶(SOD)活性、微粒体血红素氧化酶-1(HO-1)的活性以及血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)含量。结果:与酒精组比较,低、高剂量PVAR组大鼠肝脏组织ROS、MDA明显降低(P<0.05)而GSH含量显著升高(P<0.05,P<0.01);血清GPT、GOT含量明显下降(P<0.05),高剂量PVAR组SOD、HO-1活性明显升高(P<0.05)。结论:PVAR对酒精慢性中毒大鼠肝脏氧化损伤具有保护作用,其机制可能与诱导HO-1活性,清除自由基,抑制脂质过氧化反应有关。

Objective To study the protective effects of the polyphenols of vitis amurensis Rupr（PVAR） on oxidative damage in rats caused by chronic alcohol intakes.Methods Forty male Wistar rats were divided into 4 groups according rats＇ weights： normal control group,alcohol injury group（the rats were treated with 10 mL·kg^-1 33% alcohol via i.g）;PVAR group（after ingesting 200 and 400 mg·kg^-1 PVAR,the male rats were treated with 10 mL·kg^-1 33% alcohol via i.g）.After 8 weeks,the concentrations of malondialdehyde（MDA）,reactive oxygen species（ROS）,glutathione（GSH） and the activities of SOD and heme oxygenase-1（HO-1） in rat liver tissues were measured.The activities of serum glutamic-pyruvic transaminase（GPT） and glutamic oxaloacetic transaminase（GOT） in rats were measured.Results Compared with alcohol injury group,the concentrations of MDA,ROS,GPT and GOT were decreased significantly（P〈0.05）,and the GSH level was increased significantly（P〈0.01） in PVAR groups.The activities of SOD,HO-1 in PVAR groups were increased significantly（P〈0.05）.Conclusion PVAR has protective effects on oxidative damage induced by chronic alcohol intakes and the effects are related to increasing HO-1 activity,scavenging oxygen free radicals and reducing lipid peroxidation.