串联抑制素基因免疫诱导绵羊孪生的研究

Tandem Inhibin Gene Immunization to Induce Sheep Twinning

为了研究串联抑制素基因免疫对绵羊孪生的影响，以猪抑制素α（1～32）基因及绵羊补体C3d基因作为选择基因，利用RT-PCR技术构建串联抑制素重组质粒，并对60只绵羊进行免疫试验。结果表明：串联抑制素重组质粒pcDNA-DPPISS-DINH和pcDNA-DPPISS-DINH-sC3d3构建正确，并在BHK-21细胞中获得了分泌型表达。重组质粒pcDNA-DPPISS-DINH和pcDNA-DPPISS-DINH-sC3d3免疫绵羊后，双羔率分别为12.5%和25.0%，均与对照组间差异显著（P〈0.05）。串联抑制素重组质粒的成功构建，为抑制素基因疫苗的研制提供了理论依据和技术支撑。

To study the effect of tandem inhibin gene immunization on sheep twinning, the recombinant plasmid of tandem inhibin were constructed with inhibin α-subunit （1-32） of pig and complement 3d of sheep by RT-PCR, which were used to immunize the sheep. The results showed that the recombinant plasmid, pcDNA-DPPISS-DINH and pcDNA-DPPISS-DINH-sC3d3 were constructed successfully. After BHK-21 cells were transfected with the recombinant plasmid pcDNA-DPPISS-DINH and pcDNA-DPPISS-DINH-sC3d3, secreted expression was obtained. After immunization, the twinning rate of sheep was 12.5% and 25.0%, respectively, which was significantly higher （P〈0.05） than that in the control group. The construction of recombinant plasmid of tandem inhibin gene made the theoretical and technical basis for developing the inhibin gene as vaccine for sheep.