1株H5N1亚型高致病力禽流感病毒人工感染鸭的抗原定位观察

Localization of a Highly Pathogenic Avian Influenza Virus,A/duck/Guangdong/220/2004(H5N1)in Experimentally Infected Ducks

通过对鸭静脉接种或眼-鼻-口腔-泄殖腔接种禽流感病毒A/duck/Guangdong/220/2004(H5N1)后,采用免疫组织化学方法检查病毒核蛋白抗原,探讨了该病毒在组织器官中的定位与分布。结果表明,病毒核蛋白抗原主要存在于鸭的心肌细胞、肝细胞、枯否氏细胞、胰腺泡上皮细胞、肾小管上皮细胞、血管内皮细胞和血液吞噬性白细胞;在气管和支气管黏膜上皮,脑组织,肠黏膜上皮,胸腺、法氏囊、脾脏和肺脏的巨噬细胞或组织细胞以及坏死的细胞碎片中也可检查到少量病毒核蛋白抗原;而在食管、胃、睾丸、卵巢、甲状腺、肾上腺、哈德氏腺、骨骼肌等未检查到该抗原。

Using streptavidin-biotin complex immunoperoxidase staining, the tissue localization and distribution of a highly pathogenic avian influenza virus, A/duck/Guangdong/220/2004 （H5N1） were investigated in ducks inoculated by intravenous and ocular-nosal-oral-cloacal routes. The results showed that the viral nucleoprotein antigen was most often detected in the cardiac muscle cells, hepatocytes, Kupffer cells, pancreatic acinar epithelial cells, renal tubular epithelial cells, vascular endothelial cells and phagocytic leucocytes, and occasionally in the mucosa epithelium of trachea, bronchia and intestinal tract, encephalon, and macrophages, histiocytes and necrotic cellular debris of thymus, bursa of Fabricius, spleen and lung. But no viral nucleoprotein antigen was detected in the oesophagus, stomach, testis, ovary, thyroid glands, adrenal glands, Harderian glands and skeletal muscles.