4种中药贯众原植物提取物的体外抗菌活性研究

In vitro Antibacterial Activities Extracts from Four Plants Used as Traditional Chinese Medicine Guanzhong

用水提法和醇提法制备苏铁蕨(Brainea insignis)、华南紫萁(Osmunda vachellii)、狗脊蕨(Woodwardia japonica)和乌毛蕨(Blechnum orientale)的根状茎的提取液,应用纸片扩散法和试管二倍稀释法测定了各提取液对11种常见致病菌的抑菌圈、最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果表明,各提取液(1g生药/mL)对表皮葡萄球菌、枯草芽孢杆菌、金黄色葡萄球菌及李斯特菌4种革兰氏阳性菌有明显抑制作用,对表皮葡萄球菌的抑制作用尤为明显,且醇提液的抑菌效果优于水提液;对大肠埃希氏菌等革兰氏阴性菌则无抑制作用。在4种贯众原植物中,苏铁蕨对革兰氏阳性菌的抑制活性最强。

The water extracts （ PWE ） and the alcohol extracts （ PAE ） from rhizome of Brainea insignis, Osmunda vachellii, Woodwardia japonica and Blechnum orientale were prepared respectively. The inhibition zone, minimal inhibitory concentration （ MIC ） and minimal bactericidal concentration （ MBC ） of extracts to 11 kinds of common pathogenic bacteria were determined by disk diffusion method and tube double dilution method. The results showed that the PWE and the PAE of all those plants（ 1 g crude drug/mL） had obviously inhibitory effect on four Gram-positive bacteria, Staphylococcus epidermidis, Bacillus subtilis, Staphlococcus aureus, and Listeria monocytogenes, especially on Staphylococcus epidermidis, and the antibacterial activities of alcohol extracts were higher than those of water extracts, but had no inhibitory effect on Gram-negative bacteria. Among the four original Guangzong plants, the extracts of Brainea insignis showed the highest inhibitory effect on Gram-positive bacteria.