采用HPLC-TOF/MS对中药复方小柴胡汤中化学成分的快速分析鉴别

HPLC-TOF/MS in rapid identification of chemical compositions in Xiaochaihu decoction

目的:采用高效液相色谱-高分辨飞行时间质谱(HPLC-TOF/MS)对中药复方小柴胡汤中的化学成分进行快速鉴别。方法:色谱柱Agilent ZORBAXSB-C18(100mm×3.0mm,3.5μm),甲醇与0.1%的甲酸水溶液梯度洗脱,柱温为25℃,进样体积4μl,流速0.6ml/min,检测器为飞行时间质谱,ESI离子源,质量数扫描范围m/z200～1500。结果:一次性鉴别出小柴胡汤中38个化学成分。结论:采用HPLC-TOF/MS对小柴胡汤中的化学成分进行了色谱表征,一次性在一张图谱上共表征出38个化学成分,这可为中药复方小柴胡汤中化学成分的体内代谢及作用机制的深入研究奠定基础。

Objective：To rapidly identify the chemical components in a traditional Chinese herb Xiaochaihu decoction by high performance liquid chromatography-time of flight mass spectrometry （HPLC-TOF/MS）.Methods：An Agilent ZORBAX SB-C18 column （100 mm×3.0 mm,3.5 μm） was used for rapid separation and identification of chemical components in Xiaochaihu decoction,with a mobile phase of methanol and 0.1% formic acid aqueous solvent in gradient elution.The flow rate was 0.6 ml/min and the injection volume was 4 μl.The detector was time-of-flight mass spectrometer with an ESI ion source.Scanning mass range was m/z 200-1 500.Results：Thirty-eight chemical compounds were identified from Xiaochaihu decoction in one run.Conclusion：Thirty-eight chemical compounds have been identified in Xiaochaihu decoction by HPLC-TOF/MS in one run,which paves a way for further understanding the metabolism and mechanism of the chemical compounds in Xiaochaihu decoction.