肺鳞状细胞癌中人类乳头瘤状病毒16/18感染和Bcl-2、Bax蛋白表达的关系

The Relationship between the Status of Human Papillomavirus 16/18 Infection and the Expression of Bcl-2 and Bax in Squamous Cell Carcinomas of the Lung

背景与目的病毒和肿瘤的关系越来越引起人们的重视,且我国可能是HPV的高感染区,肺癌中是否存在HPV感染以及其是否与肺癌的发生发展有关仍然未明。本研究旨在探讨肺鳞状细胞癌中HPV16/18型感染状态和Bcl-2、Bax蛋白表达的关系。方法采用原位杂交检测肺鳞状细胞癌中HPV16/18DNA感染状态,应用免疫组化检测Bcl-2、Bax蛋白表达。结果44例肺鳞状细胞癌中,HPV16/18型23例(52.27%)呈整合状态,9例(20.45%)呈大部分游离而少许为整合状态,12例(27.27%)阴性。15例非肿瘤肺组织标本中未检测到HPV16/18型,差异有统计学意义(P<0.001);Bcl-2蛋白20例(45.5%)阳性,其表达与HPV16/18在癌细胞中的存在状态有关(P<0.05)。结论HPV感染可能是部分肺鳞状细胞癌的致癌因素之一,在这部分癌中Bcl-2的表达显著高于HPV阴性和存在游离状态HPV的病例。

Background and objective The relationship between the virus and carcinomas has attracted attention and China may be highly infected-District with HPV. It was unclear that whether there has been HPV infection in squamous cell carcinomas and the occurrence and development of lung cancer has the relation with HPV. This experiment applied situ hybridization and immunohistochemical techniques. This study is to investigate the relationship between the status of HPV16/18 infection between the expression of Bcl-2 and Bax in squamous cell carcinomas of the lung. Methods In 44 patients with squamous cell carcinomas of the lung, HPV 16/18 DNA was examined by in situ hybridization, expression of Bcl-2 and Bax was revealed by immunohistochemistry and koilocytes was detected in carcinomas tissues by morphologic examination. Results Of 44 patients with squamous cell carcinomas of the lung, 12 （27.27%） were found to be HPV negative. Twenty-three （52.27%） were found to be integrated form HPV, 9 （20.45%） were found to be large number of episomal form and a few integrated form HPV. And no simplex episomal form HPV was found. HPV 16/18 DNA could not be detected in 15 non-carcinomas tissues. A significantly higher 16/18 DNA positive rates in carcinomas tissues compared to non-carcinomas tissues （P〈0.001）. Of 44 patients, 20 （45.5%） were found to be Bcl-2 positive. There were significantly difference between integrated and other form HPV 16/18 in expression of Bcl-2 （P〈0.05）. Conclusion HPV infection might be a particular agent in patient with squamous cell carcinomas of the lung. The levels of expression of Bcl-2 were significant higher in HPV positive patients than patients with HPV negative or episomal form HPV in squamous cell carcinomas of the lung.