摘要
目的:利用蔓越莓抑制P菌毛阳性大肠埃希菌对P血型红细胞黏附作用的体外模型,检测中国(国产1组和2组)和美国(美国组)3个不同产地蔓越莓制剂抑制细菌对红细胞的黏附作用。方法:以P菌毛阳性大肠埃希菌与人P血型红细胞结合引起的血凝现象为基础,辅助染色标本和显微镜镜下观察,分别检测蔓越莓在水制剂中和二甲基亚砜(DMSO)中的血凝抑制作用。结果:美国产蔓越莓水制剂和DMSO制剂最低有效血凝抑制浓度分别为11.25g/L和5.11g/L;国产1组蔓越莓水制剂和DMSO制剂最低有效血凝抑制浓度分别为9.38g/L和17.04g/L;国产2组蔓越莓DMSO制剂最低有效血凝抑制浓度为20.45g/L,而其水制剂在体外实验中未见有效的血凝抑制作用。结论:不同产地的蔓越莓制剂对P菌毛阳性大肠埃希菌引起的血凝现象均有不同程度的抑制作用,提示蔓越莓制剂具有不同程度的抑制P菌毛阳性大肠埃希菌对细胞的黏附及致病作用。
Objective:To investigate the effects of anti-adhesion activity of different cranberry products grown in China and United State by establishing a cell model of P-fimbriated Escherichia coli (P*-E.coli) from epithelial cells in vitro. Methods: Based on the hemagglutination caused by P-fimbrial adhesion of P*E.coli with P blood group's erythroeytes, antiadhesion activity of cranberry product was detected by bacterial hemagglutination inhibition (HGI) test in vitro. Results: The minimum effective concentrations of HGI were 11.25 g/L in water solution and 5.11 g/L in DMSO solution for the cranberry products in USA group, and 9.38 g/L in water solution and 17.04 g/L in DMSO solution in domestic group 1, and 20.45 g/L of DMSO solution in domestic group 2. But bacterial HGI was not detected in water solution of domestic group 2. Conclusion: The results suggested that the cranberry products from China and USA may enhance anti-adhesion activities for P*-E.coli in vitro. This study demonstrated the effective inhibition of bacterial hemagglutination by domestic-original cranberry products in vitro.
出处
《天津医药》
CAS
北大核心
2009年第9期743-746,I0001,共5页
Tianjin Medical Journal
关键词
纤毛
细菌
大肠杆菌
红细胞
血型抗原
蔓越莓
fimbriae, bacterial Escherichia coli erythrocytes blood group antigens Cranberry