蛋白激发子PeaT1的高密度发酵及生理功能检测

High Cell Density Fermentation of Protein Elicitor PeaT1 and Its Physiological Function Detection

PeaT1是从极细链格孢菌(Alternaria tenuissima)中提取出的一种植物蛋白激发子,具有促进植物生长,增强作物抗逆性的功能。由于该蛋白具有无残留、无毒副作用的优点,因此具有发展为生物农药的应用前景。在100L发酵罐中利用分批补料技术对PeaT1工程菌进行高密度发酵,通过AKTA蛋白纯化仪对发酵产物进行了亲和纯化,并检测了纯化所得蛋白对水稻在15℃低温下生长的影响。发酵最终菌体密度达80g/L,每升菌液纯化得到蛋白90mg,纯化所得蛋白能够促进水稻低温下的生长,具有激发子活性。

PeaT1 is a protein elicitor obtained from Alternaria tenuissima. It was found that this protein had the function of promoting plant growth and increasing the tolerance of crops. It could be developed as a biological pesticide without residual, and effect characters. In this paper, the engineering bacteria of PeaT1 was used for high cell density fermentation. The expressed protein was purified by affinity chromatography. The final cell density reached 80 g/L and about 90 mg/L purified PeaT[ could be gained. The function assay indicated that PeaTl could promote rice growth at 15℃ temperature. This research could promote the development of PeaTI as biological pesticide.