摘要
利用２，４－Ｄ和６－ＢＡ２种外源激素对烟草未授粉子房单倍体进行了直接途径和间接途径的诱导。结果表明，２，４－Ｄ能诱导子房通过间接途径形成胚状体；６－ＢＡ浓度为２２２μｍｏｌ·Ｌ－１和４４４μｍｏｌ·Ｌ－１时，胚状体的形成为间接途径，浓度为８８９μｍｏｌ·Ｌ－１时则为直接途径。在一定用量范围内，２种激素都有随浓度的增加胚状体诱导率提高的趋势。２种激素不同浓度配合使用，其诱导途径有一定差异，当２，４－Ｄ浓度为２２６μｍｏｌ·Ｌ－１，６－ＢＡ浓度为１７７８μｍｏｌ·Ｌ－１时胚状体的诱导率最高。
The effects of exogenous hormone on haploid plant production from unpollinated ovaries of Nicotiana tabacum L.were investigated.The results obtained were summarized as follows:there were two ways in haploid-induction:the direct way and the indirect way.Embryoid could be induced by indirect way when the concentration of 6-BA were 2.22 μmol·L-1 and 4.44 μmol·L-1.But it would be by direct way if 6-BA was 8.89 μmol·L-1.The induction frequency of embroid increased when the concentration of 2,4-D or 6-BA was raised.With different conbination of 2,4-D and 6-BA,the way of induction would not be the same.The highest induction frequency of embroid (86.67%) could be obtained when supplemented with 2.26μmol·L-1 2,4-D and 17.78 μmol·L-1 6-BA.
出处
《河南农业大学学报》
CAS
CSCD
1998年第2期167-170,175,共5页
Journal of Henan Agricultural University
关键词
外源激素
烟草
未授粉子房
组织培养
诱导率
exogenous hormone
Nicotiana tabacum L.
unpolliated ovary
tissue culture
haploid-induction
