罗格列酮对同型半胱氨酸诱导大鼠肾脏微血管内皮细胞MCP-1和ICAM-1表达的影响

Rosiglitazone Inhibits the Expression of MCP-1 and ICAM-1 Induced by Homocysteine in Renal Microvascular Endothelial Cells

目的了解同型半胱氨酸（Hcy）对肾脏微血管内皮细胞（RMECs）、单核细胞趋化蛋白1（MCP-1）和细胞间黏附分子1（ICAM-1）表达的影响及罗格列酮的干预作用。方法将原代培养的大鼠RMECs分为空白对照组、Hcy刺激组和罗格列酮干预组。分别采用免疫荧光法和酶联免疫吸附试验检测RMECs表达MCP-1、ICAM-1的能力。结果不同浓度的Hcy刺激后，ICAM-1的水平显著增加（P〈0.05），且呈时间和剂量依赖性，尤其在0.1 mmol/L Hcy刺激24～48 h时达高峰。罗格列酮干预后ICAM-1含量显著降低，且随罗格列酮浓度的增加其抑制作用更加明显；0.1 mmol/L的罗格列酮干预24h后，ICAM-1的水平与空白对照组比较，差异无统计学意义（P（0.05）；MCP-1荧光强度在罗格列酮干预后也减弱。结论罗格列酮能抑制Hcy诱导的RMECs表达MCP-1和ICAM-1。

Objective To investigate the effect of rosiglitazone on the expression of monocyte chemoattractant protein-1 （ MCP-1 ） and intercellular adhesion molecule-1 （ ICAM-1 ） induced by homocysteine（Hcy） in renal microvascular endothelial cells（RMECs）. Methods Rat RMECs by primary culture were divided into three groups：blank group, Hcy stimulate group and rosiglitazone intervention group. The expression of MCP-1 ,ICAM- 1 in RMECs was tested by Immunofluorescence and enzyme linked immunosorbent assay respectively. Results The level of ICAM-1 were added significantly（ P 〈 0.05 or P 〈 0.01 ） with various concentrations of Hcy, and increased following time and concentrations.The level of ICAM-1 arrived at peak during 24 h to 48 h with 0.1 mmol/L Hcy. The level of ICAM-1 was decreasd significantly, and the higher the concentration of rosiglitazone was,the stronger the in hibitory action was. The level of ICAM-1 in 0.1 mmol/L rosiglitazone group compared with that in blank group was no statistically significance. The fluorescence intensity of MCP-1 was weaken with rosiglitazone. Conclusion Rosiglitazone can inhibit the expression of MCP-1 and ICAM-1 induced by homocysteine in RMECs.