ELISA技术检测马铃薯病毒的研究

Application of ELISA Technique for Determination Potato Virus

应用ＤＡＳＥＬＩＳＡ方法对甘肃省种植的６个马铃薯品种老龄薯和幼龄薯及２个品种的茎尖组培苗进行了针对ＰＶＸ、ＰＶＹ及ＰＬＲＶ的病毒检测。结果表明，老龄薯块携带以上３种病毒，并以ＰＬＲＶ为主体的复合侵染为主，幼龄薯块仅带其中一种病毒，且带毒量少；茎尖剥离组培法可使陇薯３号和１６７４５脱毒率达２０％。以幼龄薯为脱毒基础材料可望进一步提高脱毒率。文中还对ＤＡＳＥＬＩＳＡ技术在马铃薯病毒检测中的优势进行了评价。

Both ripe and young potatoes of 6 cultivars planted in Gansu and meristem tip culture seedlings of 2 cultivars are tested to determine PVX, PVY and PLRV using DAS ELISA method. The results show that all of ripe potatoes carry all of the 3 viruses infected mainly through complex pattern dominated by PLRV. The young potatoes carry only one of the viruses and low grade infection is found. The rate of virus free seedlings reaches 20 %, which is obtained by means of meristem tip culture. It indicates that using young potato as virus free material can be expected to obtain totally virus free seedlings. The advantage of DAS ELISA method was also evaluated applied to determine potato virus in the paper.