结肠癌SW480细胞FAP-1表达与奥沙利铂化疗耐药关系的研究

Study on the relationship between the expression of FAP-1 of colon carcinoma cells and oxaliplatin chemo-resistance

目的探讨结肠癌FAP-1表达与其对奥沙利铂化疗耐药间的关系。方法用奥沙利铂处理结肠癌SW480细胞,用MTT法评价其细胞增殖能力,以RT-PCR法评价FAP-1 mRNA表达水平。结果奥沙利铂化疗仅能一定程度上抑制结肠癌SW480的增殖,但随作用时间的延长,SW480细胞仍能继续增殖。经过奥沙利铂化疗FAP-1的表达上调。结论奥沙利铂化疗可引起FAP-1表达的上调,增强肿瘤细胞对抗Fas诱导的细胞凋亡。这可能与结肠癌对奥沙利铂化疗的耐药相关。

Objective To investigate the relationship between FAP-1 expression and the chemo-resistance to Oxaliplatin of human colorectal cancer ceil line SW480. Methods Mter Oxaliplatin administration, Methyl thiazolyl tetrazolium （MTT） assay was used to measure proliferation of SW480 ceils, RT-PCR was used to measure the FAP-1 mRNA lever. Results After oxaliplatin treatment, the proliferation of SW480 ceils could be reduced to a low degree, but as time went by, the SW480 cell could still proliferate. Oxaliplatin can up-grate FAP-1 expression in SW480. Conclusion Oxaliplatin could enhance the expression of FAP-1 of SW480 cells, thus, enhance the anti-Fas induced apoptosis of the tumor cells. This may account for the chemo-resistance to Oxaliplatin of colorectal cancer.