蛋白激酶受体在炎症性肠病大鼠模型结肠组织中表达

Expression of protease-activated receptors in colon tissue of rat model with trinitrobenzene sulfonic acid-induced inflammatory bowel diseases

目的研究TNBS诱导IBD的大鼠模型制备以及了解IBD大鼠的结肠组织中PAR受体家族存在的情况。方法肛门灌注TNBS用7天造模。7天后时疾病活动情况及;组织学损伤的评估;采用髓过氧化物酶活性试验以及实时定量荧光PCR检测方法。结果实验7天时两组体重比较分别为239±17.77克;161.5±5.19克,P<0.05。实验组体重减少22.9±4.6克;对照组增加60.65±14.63克;实验期间每日两组大鼠进食为5.4±2.0克,23.46±4.1克;P<0.01。TNBS-组大鼠DAI评分明显低于对照组大鼠的平均DAI评分分别为8.8分和0.2分。对照组1.08±0.09U/1cm结肠;P<0.05。各实验组大鼠上述两种PAR-1 mRNA,PAR-2 mRNA均显著上升,分别为317.0±53.86%,354.9±97.48%。相比对照组大鼠的PAR-1 mRNA,PAR-2 mRNA水平为81.25±13.30%;83.50±524.42%,P<0.05。结论TNBS可以很好的制备出IBD大鼠模型。TNBS诱导的IBD大鼠模型的结肠组织中存在着PAR-1 mRNA和PAR-2 mRNA水平升高,提示着PARs受体的激活在IBD的炎症过程起着一定的作用。

Objective To examine the expression of PAR-lmRNA and PAR-2 mRNA level in colon tissue and foundation of Rat Model with TNBS-induced IBD. Methods After an overnight fasting, all animals were enemaed into the colon by TNBS. Mice were sacrificed at specific days as outlined below. After recording the body weight, and its length （cm） and weight （rag） recorded. Examine the expression of PAR-lmRNA and PAR-2 mRNA level in colon tissue were measured after foundation of rat model with TNBS-induced IBD. Result After 7 days of administration, the TNBS- induced IBD rat model showed a higher DAI （8.8 vs. 0.2）, a higher histological score （6.3 vs. 1.3）. There were significant differences in PAR-lmRNA and PAR-2 mRNA level in colon tissue between the two groups. Conclusions TNBS can successfully induced IBD rat model. TNBS-induced IBD rat model of the colon tissue of the existence of increased PAR-1 mRNA and PAR-2 mRNA levels, suggesting that activation of PARs receptor in IBD the inflammatory process plays a role.